RAM 2000 BU Series Manual de usuario descargar pdf (Pagina 5)

R. KULAKSIZ, Ç. ÇEBİ, E. AKÇAY

181

Di erences among the extenders in terms of

spermatozoa motility and total abnormality were

found to be signi cant post-thawing.  e data on

motility and abnormality post-thawing in the T,

SC, M, and B extenders are presented in Table 2.

Spermatoza motility was signi cantly higher (P

< 0.05) in M (21.2%) compared with B (7.0%), SC

(11.5%), and T (16.2%) a er being thawed.  e total

abnormality of semen diluted in M (50%) was lower

than that observed in the other extenders.

Discussion

From the present results it was observed that the type

of extender used in diluting Akkaraman ram semen

is an important factor in the successful preservation

(at 4 °C in the refrigerator) and cryopreservation (at

−196 °C in liquid nitrogen) of ram spermatozoa.

Many extenders have been used for freezing ram

semen and reports show that the type of extender

used plays an important role in the successful

preservation of ram semen (11-13). Skimmed milk

extenders preserve sperm motility better than other

extenders.  ese  ndings were in agreement with

the results of Lopez-Saez et al. (13) and Kulaksız

et al. (14) on ram semen. Contrary to our results,

however, Paulenz et al. (15) and Gündoğan (16)

reported better spermatozoa motility and membrane

integrity rates in a Tris-based extender than in the

sodium citrate and skimmed milk extenders. Lopez

et al. (17) observed no di erences between sodium

citrate-, Tris-, and milk-based extenders when

subjected to liquid storage at 4 °C.  e post-thawing

sperm motility (16.2%) determined for the Tris

extender used in our study was close to the  ndings

of Tekin et al. (18) but Soylu et al. (19) reported better

spermatozoa motility and membrane integrity in Tris

extender than that found in our study.

To our knowledge, no previous study is available

on the liquid preservation of ram semen in prepared

extenders containing soy lecithin (Bioxcell) as an egg

yolk substitute. In the present study, sperm motility

showed higher values for Bioxcell compared to Tris

and sodium citrate extenders as the liquid of storage.

However, the parameters used for semen quality

in this study showed signi cantly lower values for

Bioxcell post-thawing in comparison with the other

extenders. However, Gil et al. (20) found that Bioxcell

was not di erent from a skimmed milk extender for

the freezing of ram semen.

It is most likely that the cryogenic damages are due

to the irreversible destruction of single components

of the structural organization of sperm cells. Sperm

are subjected to major changes in osmotic pressure

during freezing and thawing.  e stress on sperm

membranes is dependent upon the basic extender

used and the concentration of cryoprotectant as

they interact with the freezing and thawing rates

(21-23). Moreover, in the present study, the portion

of ejaculates collected during a non-mating season

(summer) had lower motility values and a higher

percentage of total abnormal spermatozoa in all of

the extenders a er freezing.

In conclusion, the freezability of Akkaraman ram

semen was adversely a ected by the summer term

in which the samples were collected.  e skimmed

milk extender showed the best liquid storage and

post-thaw motility compared to the other extenders.

Regardless of the extender type, however, semen

freezing increased the rate of sperm morphological

abnormalities.

Table 2.  e mean percentage of motility and total abnormality

of ram spermatozoa a er freeze-thawing in 4 di erent

extenders.

Extenders

Motility

(%)

Total abnormality

(%)

T 16.2 ± 0.2

55.4 ± 0.5

M 21.2 ± 0.1

50.6 ± 0.8

B 7.0 ± 0.1

65.0 ± 0.5

SC 11.5 ± 0.2

60.0 ± 0.4

Groups with di erent letters (a, b, c) in the same column are

signi cantly di erent (P < 0.05).

T: Tris

M: Skimmed milk

B: Bioxcell

SC: Sodium citrate

1 2 3 4 5 6

Research Article 1
References 6

Comentarios a estos manuales

Sin comentarios

RAM 2000 BU Series Manual de usuario Pagina 5

Comentarios a estos manuales

Relacionado con productos y manuales para Amplificadores de audio RAM 2000 BU Series